загрузка...
 
The comparative analysis of 17 cytokines level in serum and whole blood of healthy donors using the Bio-Plex protein assay system
Повернутись до змісту

The comparative analysis of 17 cytokines level in serum and whole blood of healthy donors using the Bio-Plex protein assay system

A.A. Ostanin, E.R. Chernykh Institute of Clinical Immunology, Siberian Branch of RAMS, Novosibirsk

The purpose of this study was to evaluate the level of 17 cytokines (IL-1p, TNFa, IFNy, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17, G-CSF, GM-CSF, MCP-1, MIP-ip) in serum and spontaneous and mitogen-induced (LPS, ConA) whole blood of healthy donors and to determine the normal values of human cytokine status. The marked inter-individual variation in serum level of IFNy, IL-13, IL-1 p, TNFa, MIP-1p, G-CSF and GM-CSF, and the significant negative age-related correlation with serum concentration of IFNy, TNFa, IL-12, IL-7 and GM-CSF were revealed. The serum levels of pro- inflammatory (IL-1p, TNFa), Th1 (IFNy) and Th2 cytokines (IL-13), as well as the growth factors (IL-7, GM-CSF) were found to be considerably decreased in older donors (> 43 years, mean 48) compared to healthy subjects at the age from 20 to 30 years (mean 24). It has been shown that the measurement of spontaneous and mitogen-stimulated cytokine production in whole blood cultures essentially up-grade the diagnostic value of cytokine status analysis. The obtained data of in vivo and ex vivo cytokines level, in detail presented as M ± S.E., median and min-max range, may be used as normal data-base in subsequent investigations. (Cytokines and Inflammation. 2005. Vol. 4, № 2. P. 25-32.)

Key words: human cytokines, serum, whoLe bLood, Bio-PLex.

ОРИГИНАЛЬНЫЕ СТАТЬИ

© Коллектив авторов, 2005

УДК 612.08:612.017.1   



загрузка...