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PRACTICAL WORKS Practical work #1. Definition of hematocrit index
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PRACTICAL WORKS Practical work #1. Definition of hematocrit index

Hematocrit index (hematocrit) characterizes the percentage volume of formed elements (erythrocytes) in blood. There are venous, capillary and arterial hematocrits, because the volume of formed blood elements, especially erythrocytes, is different in different parts of the bloodstream. The lowest hematocrit is in the arterial blood.

Objectives: to define the percentage volume of formed elements (erythrocytes) in capillary blood and estimate its value.

Requirements for work: hematocrit capillary or micropipette, centrifuge with 8000 rotations per minute, plasticine, aqueous solution of heparin (with activity 5000 OD/ml), 96% alcohol, 2% alcohol solution of iodine, cotton wool.

to wash calibrated hematocrit pipette with heparin solution;

to blow pipette;

to dry pipette and fill with blood on 7/8 long;

to fix the opening of pipette with plasticine and to centrifuge for 5 minutes with 8000 rotations per minute;

to define the percentage volume of formed elements comparatively to the general blood volume, that is hematocrit value.

Recommendations for writing down the results

Write down hematocrit value in percents.

Answer following questions in conclusion:

Is the percentage volume of formed element (erythrocytes) in capillary blood normal?

What changes in blood system testify increase or decrease of hematocrit value?



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