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Elevation of TNFα and IL-6 levels in preterm human placentae compared to term placentae M. Huleihel , G. Holcberg, O. Sapir , S. Levi , M. Katz , L. Myatt , M. Mazor

Elevation of TNFα and IL-6 levels in preterm human placentae compared to term placentae M. Huleihel , G. Holcberg, O. Sapir , S. Levi , M. Katz , L. Myatt , M. Mazor

Department of Microbiology and Immunology;

Department of Obstetrics and Gynecology, Soroka University Medical Center, Faculty of Health Sciences, BenGurion University of the Negev, Beer Sheva, Israel; University of Cincinnati, College of Medicine, Cincinnati, OH, USA

Aim of the study. To determine the concentrations of TNFα and IL6 in the perfusate of isolated human placental cotyledon from term and preterm deliveries. Study design. Isolated placental cotyledons were dually perfused in 5 term placentas and 4 placentas obtained after preterm birth (2834 W of gestation). Perfusate samples from fetal and maternal sites were collected every 30 min (for 10 hours) and were examined by ELISA for IL-6 and TNFα levels. Statistical analysis was performed by ANOVA and paired t test. Results. A significant increase of TNFα and IL-6 concentrations was observed in perfusate of maternal and fetal circulation in term and preterm placentas during 10 hours of perfusion. The increase of these cytokines in perfusate was time course dependent. The TNFα levels were significantly higher in maternal compartment in both groups (421 217 and 522 179 pg/ml respectively) as compared to fetal compartment (20 9 and 65 25 pg/ml respec tively, p < 0,05). Moreover the TNFα levels were significantly higher in perfusate of preterm placentas in maternal and fetal compartments (522 179 and 65 25 pg/ml respectively) as compared to term placentas (20 9 and 421 217 pg/ml respectively, p < 0,05) after 10 hours of perfusion. The IL-6 levels were significantly higher in preterm placentae as compared to term placentae in maternal and fetal compartments (1945 688 pg/ml and 971 390 pg/ml in maternal compartment respectively, and 419 154 pg/ml and 90 39 pg/ml in fetal compartment respectively p < 0,05). Conclusions. Perfusate of maternal sites from term and preterm placentas show higher levels of TNFα and IL-6 than of the fetal sites. Perfusates of preterm placentas shows higher levels of TNFα and IL-6 than term placentas. Our results may indicate the involvement of these cytokines in the process of the preterm parturition.



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