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Distinct expression of pro$inflammatory cytokines and their modulators (IL-1Ra and IL-10) in testicular tissue cells of fertile and infertile men M. Huleihel , E. Lunenfeld, V. Dyomin, N. Vardi , I. Ynai)Inbar , L. Harosh, G. Potashnik
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Distinct expression of pro$inflammatory cytokines and their modulators (IL-1Ra and IL-10) in testicular tissue cells of fertile and infertile men M. Huleihel , E. Lunenfeld, V. Dyomin, N. Vardi , I. Ynai)Inbar , L. Harosh, G. Potashnik

Department of Microbiology and Immunology;  

Department of Obstetrics and Gynecology;  

Pathology Institute, Soroka University Medical Center, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel Introduction. Cytokines are produced and involved in the regulation of testicular cell functions. Our aim: to evaluate the levels of inflammatory cytokines (IL-1?, IL-1?, IL-6 and TNF?) and their down regulators (IL-1Ra and IL-10) in testicular biopsies of fertile and infertile men. Methods. Biopsies from 6 fertile men, 6  infertile men with  incomplete maturation arrest and 6  infertile men with Sertoli only syndrom men were examined by  immunohistochemical  staining for the expression levels and cellular localization of IL-1?, IL-1?, IL-6, TNF?, IL-1Ra and IL-10. Results see in table. Conclusions. This is the first report showing expression of immuno regulatory cytokines in human testicular tissues. IL-1?, IL-1Ra,  IL-6 and TNF? are differently expressed  in  testicular cells of pathological conditions as compared to normal. The distinct expression of the cytokines in the different testicular cells may indicate their involvement in the function of these cells. The expression of high levels of IL-10 in testicular tissues may suggest its involvement  in a general  suppression mechanism against autoimmune response against sperm cells in the testicular compartment. The mechanism by which cytokines  involved  in male  fertility  istively, p < 0,05). Moreover  the TNF?  levels were  significantly higher  in perfusate of preterm placentas  in maternal and  fetal compartments (522 ± 179 and 65 ± 25 pg/ml respectively) as compared to term placentas  (20 ± 9 and 421 ± 217 pg/ml respective ly, p < 0,05) after 10 hours of perfusion. The IL-6 levels were significantly higher  in preterm placentae as compared to term placentae in maternal and fetal compartments (1945 ± 688 pg/ml and 971 ± 390 pg/ml  in maternal compartment  respectively, and 419 ± 154 pg/ml and 90 ± 39 pg/ml in fetal compartment respec tively p < 0,05). Conclusions. Perfusate of maternal sites from term and preterm placentas show higher  levels of TNF? and IL-6 than of the fetal sites. Perfusates of preterm placentas shows higher levels of TNF? and  IL-6  than  term placentas. Our  results may  indicate  the  involvement of these cytokines in the process of the preterm parturition.



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