Distinct expression of pro$inflammatory cytokines and their modulators (IL-1Ra and IL-10) in testicular tissue cells of fertile and infertile men M. Huleihel , E. Lunenfeld, V. Dyomin, N. Vardi , I. Ynai)Inbar , L. Harosh, G. Potashnik
Distinct expression of pro$inflammatory cytokines and their modulators (IL-1Ra and IL-10) in testicular tissue cells of fertile and infertile men M. Huleihel , E. Lunenfeld, V. Dyomin, N. Vardi , I. Ynai)Inbar , L. Harosh, G. Potashnik
Department of Microbiology and Immunology;
Department of Obstetrics and Gynecology;
Pathology Institute, Soroka University Medical Center, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel Introduction. Cytokines are produced and involved in the regulation of testicular cell functions. Our aim: to evaluate the levels of inflammatory cytokines (IL-1?, IL-1?, IL-6 and TNF?) and their down regulators (IL-1Ra and IL-10) in testicular biopsies of fertile and infertile men. Methods. Biopsies from 6 fertile men, 6 infertile men with incomplete maturation arrest and 6 infertile men with Sertoli only syndrom men were examined by immunohistochemical staining for the expression levels and cellular localization of IL-1?, IL-1?, IL-6, TNF?, IL-1Ra and IL-10. Results see in table. Conclusions. This is the first report showing expression of immuno regulatory cytokines in human testicular tissues. IL-1?, IL-1Ra, IL-6 and TNF? are differently expressed in testicular cells of pathological conditions as compared to normal. The distinct expression of the cytokines in the different testicular cells may indicate their involvement in the function of these cells. The expression of high levels of IL-10 in testicular tissues may suggest its involvement in a general suppression mechanism against autoimmune response against sperm cells in the testicular compartment. The mechanism by which cytokines involved in male fertility istively, p < 0,05). Moreover the TNF? levels were significantly higher in perfusate of preterm placentas in maternal and fetal compartments (522 ± 179 and 65 ± 25 pg/ml respectively) as compared to term placentas (20 ± 9 and 421 ± 217 pg/ml respective ly, p < 0,05) after 10 hours of perfusion. The IL-6 levels were significantly higher in preterm placentae as compared to term placentae in maternal and fetal compartments (1945 ± 688 pg/ml and 971 ± 390 pg/ml in maternal compartment respectively, and 419 ± 154 pg/ml and 90 ± 39 pg/ml in fetal compartment respec tively p < 0,05). Conclusions. Perfusate of maternal sites from term and preterm placentas show higher levels of TNF? and IL-6 than of the fetal sites. Perfusates of preterm placentas shows higher levels of TNF? and IL-6 than term placentas. Our results may indicate the involvement of these cytokines in the process of the preterm parturition.