Causative agents of Botulism, Food Toxic Infection and Food Intoxications
167. Material taken from a patient suspected to have botulism was taken to bacteriological laboratory. What medium should be used for agent isolation?
+ Kitt-Tarozzi medium.
– 1% alkaline peptone water.
– Endo agar.
– Sotton medium.
– L?wenstein-Jensen medium.
168. Home made dried fish, which was the reason of severe food poisoning, was taken to bacteriological laboratory. During its investigation, tennis racket-shaped microorganisms were isolated on Kitt-Tarozzi medium. For final proof of the diagnosis it was necessary to introduce the material to white mice intraperitonially. What diagnosis will the doctor make?
– Salmonellosis.
+ Botulism.
– Cholera.
– Shigellosis.
– Typhoid fever.
169. Feces of a child with enteritis diagnosis is emulgated in physiological solution and the emulsion drop is put on selective medium: 10% milk-salt or yolk-salt agar. What microorganisms are supposed to be isolated?
+ Staphylococcus.
– E. coli.
– Streptococcus.
– Klebsiella.
– Enterococcus.
170. After eating tinned meat, a patient saw objects double, had splitting head ache, swallowing disturbance, difficult breathing, muscular weakness. He was diagnosed with “botulism”. What pathogenic factors are clinical signs connected to this disease?
– Haemolysin.
+ Exotoxin.
– Endotoxin.
– Plasmocoagulase.
– Fibrinolysin.
171. After eating tinned mushrooms, a patient had symptoms of bulbar paralysis: he saw objects double, swallowing disturbance. The preliminary diagnosis is botulism. With the help of what reaction is it possible to determine the type of toxin?
– Agglutination reaction.
– Precipitation reaction.
– Complement fixation test.
– Immunofluorescent test.
+ Neutralisation test.
172. After eating home-made tinned meat, a student had symptoms of food poisoning by Clostridium botulinum: diplopia, articulate speech disturbance and paralysis of breathing muscles. What is the cause of such botulism symptoms?
+ Neurotoxin action.
– C. botulinum invasion into intestines epithelium.
– Enterotoxin secretion.
– Endotoxic shock.
– Adenil-cyclase activation.
173. After eating a cake which had been kept on the table for 24 hours, patient had signs of acute intestinal poisoning in 8 hours. What microorganisms could cause the disease?
– Clostridium perfringens.
– Escherichia coli.
+ Staphylococcus aureus.
– Salmonella enterica.
– Clostridium botulinum.
174. Infectious diseases agents produce exotoxins with different effects of biological action. Which of these toxins are activated in gastrointestinal tract?
+ Botulotoxin.
– Haematoxin.
– Histotoxin.
– Tetanospasmin.
– Cholerogen.
175. A39-year-old patient was taken to hospital in 10 hours after eating tinned “Tourist’s breakfast”. His vomiting, feces, blood, urine, remains of the food stuff were sent for bacteriological investigation. Gram positive bacilli, making tennis rocket-shaped spores which grew (at 35°C) on blood-liver agar in anaerobic conditions, were detected in Kitt-Tarozzi medium. What microorganism is it?
+ Clostridium botulinum.
– Fusobacterium nucleatum.
– Clostridium novyi.
– Bacillus subtilis.
– Bacillus cereus.
176. During the investigation of low quality foodstuff, mobile, gram-negative bacilli with serpiginous growth with the shape of coal dust on MPA after 18-hours of cultivation were detected. Isolates didn’t ferment lactose, mannitol, but fermented glucose, maltose and sucrose producing acid and gas, hydrogen sulphide and indole. It was proved bacteriologically that the identified bacteria were representatives of what genus?
– Escherichia.
+ Proteus.
– Pseudomonas.
– Salmonella.
– Shigella.
177. An outbreak of food toxic infection was registered in a town after eating boiled cakes. From the remains, pathogenic microorganisms were isolated. Which of the following microorganisms could most probably cause the given disease?
– Clostridium perfringens.
– Escherichia coli.
– Salmonella typhimurium.
+ Staphylococcus aureus.
– Salmonella enterica.
178. Inwater of Canada lakes, Clostridium botulinum was isolated in great quantity and in the given focus mass death of waterfowl was observed. What kind of infection is it?
+ Sapronosis.
– Anthroponosis.
– Zoonosis.
– Anthropozoonosis.
– Nosocomial.
179. During bacteriological investigation of vomiting, stomach washing waters and the remains of food, gram positive bacillus with rounded ends, polymorph, slow-mobile, anaerobic were isolated. Microorganism makes colonies of irregular form with extensions on glucose-blood agar, causes erythrocytes haemolysis and produces exotoxin. What agent was isolated?
– Proteus vulgaris.
– Bacteroides fragilis.
– Clostridium perfringens.
+ Clostridium botulinum.
– Salmonella typhimurium.
180. It is necessary to make urgent botulism prophylaxis to a patient after eating infected product. What preparation should be used for this aim?
– Placenta gamma globulin.
– Monovalent botulinic antitoxic serum.
+ Polyvalent botulinic antitoxic serum.
– Toxoid.
– Interferon.
181. After eating home-made tinned vegetables, 2 cases of poisoning, characterised by CNS infection (seeing objects double, swallowing pain, aphonia) were recorded in a family. Name the probable food intoxication agent.
– Staphylococcus.
+ Botulism agent.
– Shigella.
– Salmonella.
– E. coli.
182. Inkindergarten, after eating cottage cheese, a disease characterised by acute nausea, vomiting, diarrhoea was registered. During microscopy of preparation, made of cottage cheese and vomiting, gram positive microorganisms in form of cluster grape-shaped were detected. What are your further actions for determination of the aetiology of this food intoxication outbreak?
+ To make further bacteriological investigation.
– To determine antibodies in blood serum additionally.
– To make an additionall allergic test.
– To conclude that staphylococcus was the reason of the disease.
– To make investigation of kitchen equipment.
183. During identification of food toxic infection agent, it was determined that the agent belongs to Salmonella genus according to biochemical properties. What agent feature will allow determining this specific belonging most precisely?
+ Antigen structure.
– Study sensitivity to antibiotics.
– Pathogenesis for different species of laboratory animals.
– Serological type of agent toxin.
– Phage typing.
184. Inkindergarten, short time after eating cottage cheese almost all children had signs of gastroenteritis. During bacteriological investigation of vomiting and cottage cheese, remnants of Staphylococcus aureus were isolated. What investigation should be made for elaboration of cause of infection?
+ To make the phage typing of isolated strains.
– To determine strain ability of toxin creation.
– To make investigation of kitchen equipment.
– To investigate antibodies presence in ill children.
– To make an allergic test.
185. Apatient was taken to a hospital with complaints of vomiting, nausea, seeing objects double, swallowing difficulty. A doctor suspected botulism. What methods of diagnostics should be used to confirm the diagnosis?
+ Biological test, bacteriological method.
– Allergic test, serological method.
– Bacteriological, mycological methods.
– Protozoological, microscopic methods.
– Bacteriological, microscopic methods.
186. Bacteriological investigation of foodstuffs (tinned) quality established the presence of microorganisms, which made spores and were botulism agents. Characterise the main properties of C. botulinum.
+ Tennis rocket-shaped microorganism because of subtermal spores arrangement.
– C. botulinum is obligate aerobe bacteria.
– C. botulinum forms a capsule in living organisms.
– C. botulinum doesn’t produce exotoxin.
– C. botulinum is a mobile gram negative bacillus.
187. During bacteriological investigation of soured cream, samples of S.aureus culture were isolated. How can the aetiological meaning of isolated culture of S. aureus be proved as food poisoning agent, which appeared among sour cream consumers?
+ Phage type determination.
– Plasmocoagulase activity determination.
– Haemotoxins determination.
– Saccharolytic properties determination.
– Lecithinase determination.
188. Inbacteriological laboratory, the investigation of tinned meat as to botulism toxin presence is being made. For this reason the extract from the investigated material and antitoxic A, B, E sera against botulism were introduced to experimental group of mice. An extract without serum was introduced to control group of mice. What serological reaction was used?
+ Neutralisation test.
– Precipitation reaction.
– Complement fixation test.
– Agglutination reaction.
– Immune double diffusion technique.
189. A35-year-old patient is complaining of progressing muscular weakness, sight deterioration, seeing objects double, “net” in front of eyes, hard food swallowing disorder and thirst. 24 hours before the disease he drank alcohol, ate tinned mushrooms. What preliminary diagnosis will you make?
+ Botulism.
– Salmonellosis.
– Mushrooms poisoning.
– Methyl spirit poisoning.
– Escherichiosis.
190. Adoctor suspected a patient with signs of food toxic infection, to have botulism. It became clear from the antecedent history that the poisoning took place during the jubilee celebration on which tinned mushrooms and fish were eaten. What biological preparation should be used for urgent botulism prophylaxis?
+ Antibotulinic serum.
– Antibotulinic tetratoxoid.
– Antibotulinic tritoxoid.
– Eubiotics.
– Gamma-globulin.
191. During the examination of a patient suspected to have food toxic infection, a doctor on duty noticed symptoms of botulism. The patient remembered the dishes he ate the day before. What foodstuffs might have caused the patient’s infection by botulism agent?
+ Home-made tinned meat.
– Boiled cakes of private producer.
– Sour cream from the local milk plant.
– Wild strawberries from dacha plot.
– Fried dietetic eggs.
192. Apatient is taken to infectious department with ”botulism” as preliminary diagnosis. What reaction should be used for isolated material for investigation of botulinic toxin?
+ Neutralization test.
– Agglutination reaction.
– Complement fixation test.
– Precipitation reaction.
– Immunofluorescence test.
193. A56-year-old male was taken to hospital with disturbance muscle of eye coordination, swallowing and articulate speech. These symptoms have appeared to the patient in 24 hours after eating home-made tinned vegetables. What preparation is necessary to be administered urgently to the patient?
+ Antibotulinic polyvalent serum.
– Botulinic toxoid types A, B, Å.
– Specific serum for neutralization test.
– Antihistamine preparation.
– Donor ?-globulin.
194. Home made dried fish, which was the reason of severe food poisoning, was taken to bacteriological laboratory. Symptoms of disease specify action strong exotoxin which involve nucleus of medulla oblongata. What diagnosis did the doctor specify in a laboratory request form to an investigated material?
+ Botulism.
– Salmonellosis.
– Dysentery.
– Cholera.
– Typhoid fever.
195. After eating cakes that were kept a day at room temperature, a patient showed signs of acute food poisoning. From pastries, provided pure culture of gram positive cocci. The selected culture on blood agar gave a hemolytic zone; when cultured in yolk-salt agar showed bacteria lecithinase activity; on milk-salt agar light Pigmented colonies formed, and in 2 hours caused coagulation of plasma. What pathogen caused the disease?
+ Staphylococcus aureus.
– Staphylococcus epidermidis.
– Staphylococcus saprophyticus.
– Salmonella typhimurium.
– Salmonella enterica.
196. Inbacteriological laboratories delivered materials (washing water, stockfish home) suspected of existence of causal agent of botulism. What fertile ground is necessary to make the primary seed material?
+ Average Kitt-Tarozzi.
– Blood-sugar-agar.
– Sugar agar.
– Sugar broth.
– Serum agar.
197. Inbacteriological laboratories were delivered canned vegetables, which have more than one person caused botulism. What conditions of cultivation should be followed to identify the causative agent in the target material?
+ Cultivated in the absence of oxygen.
– Add antibiotics that oppress the growth of gram-negative microorganisms.
– Create in nutrient alkaline reaction.
– Cultivate the crop at a temperature below 35°C.
– Add vitamins and aminoacids.
198. From kindergarten, pediatric infectious department hospitalized several children who reported vomiting, high temperature, liquid stools. The disease began 3 hours after lunch. At lunch the children ate mashed potatoes with sausages. Which disease can be suspected in the children?
+ Food borne diseases.
– Dysentery.
– Cholera.
– Colienteritis.
– Typhoid fever.
199. `Students of junior classes during break ate cake with cream and candy. After some time, most children had nausea, abdominal pain, and diarrhea. Which agent caused poisoning of the children?
+ Staphylococcus aureus.
– Pseudomonas aeruginosa.
– Candida albicans.
– Streptococcus pyogenes.
– Neisseria catarrhali.
200. `The patient with suspected foodborne diseases was diagnosed with "botulism." What should be the first steps for the doctor? What preparations are needed to appointed to the patient for treatment?
201. `Food poisoning came from several people, marking anniversary with a festive table. Cream cake was prepared with the use of duck eggs. Which genus would most likely include the bacterium causing the disease?
+ Salmonella.
– Shigella.
– Clostridia.
– Yersinia.
– Corynebacteria.
202. `Feces of a child who was diagnosed with "enteritis", bacteriologist reconstituted in saline solution, and a drop of suspension grown in 10% yolk-salt agar. What enzyme is found in the pathogenicity of this environment?
+ Lecithinase.
– Colicines.
– Streptokinase.
– Hemolysin.
– Hyaluronidase.
203. `8-month child has bowel dysfunction. Qualitative and quantitative analysis of intestinal micro flora allowed diagnosis of dysbacteriosis. For treatment the child was assigned a number of coli proteus bacteriophage. What is the mechanism of action of this drug?
+ It is conditional lysis of opportunistic enterobacteria.
– Contribute Reproduction of bifidobacteria.
– Enhances antagonistic activity lactobacilli.
– It stimulates the synthesis of secretory IgA (sIgA).
– Improves barrier properties of mucosal ulcers.
204. `With the identification of the causative agent of food diseases found that the biochemical properties of agent belongs to the genus Salmonella. What is the causal agent of the property shows its species?
+ Antigens structure.
– Pathogenicity for laboratory animals.
– Phage typing.
– Culture properties.
– Morphological and tinctorial properties.
205. `During food poisoning bacteriologist identified the culture of anaerobic spore-gram positive bacteria. These properties most likely mean which species?