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Practical work #15. Definition of the total time of blood clotting
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Practical work #15. Definition of the total time of blood clotting

Objectives: to define the total time of blood clotting and estimate it.

Requirements for work: glass, glass hook, clock, needle, 96% ethanol, 2% alcohol solution of iodine, cotton wool.

take blood from a rat’s tail, put it on the glass and check the time.

take out the content of drop with the interval 20-30 sec (hook should be held vertically), wait, till fibrin fiber will drag after the hook.

check the time again and consider it as the moment the clotting started.

put the hook into the blood, drag the drop on the glass horizontally with the same interval. Check the time as soon as clot will be dragged after the hook, which corresponds to the end of clotting.

Recommendations for the writing down the results:

Write down the time the clotting started and the time the clotting ended.

Answer following questions in conclusion:

Is the time of blood clotting normal? What does it testify?

Appendix I



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